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5 edition of Cell-based microarrays found in the catalog.

Cell-based microarrays

Ella Palmer

Cell-based microarrays

methods and protocols

by Ella Palmer

  • 25 Want to read
  • 28 Currently reading

Published by Humana Press in New York .
Written in English

    Subjects:
  • Biochips

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Ella Palmer
    SeriesSpringer protocols, Methods in molecular biology -- 706, Springer protocols, Methods in molecular biology (Clifton, N.J.) -- v. 706.
    Classifications
    LC ClassificationsR857.B5 C385 2011
    The Physical Object
    Paginationx, 209 p. :
    Number of Pages209
    ID Numbers
    Open LibraryOL25054968M
    ISBN 101617379697
    ISBN 109781617379697
    LC Control Number2011282746
    OCLC/WorldCa668191295

    In selective patterning, the abrupt expansion in the cross-sectional geometry of a microchannel at capillary barriers results in a confined advancement of the liquid–air meniscus. This protocol describes a detailed procedure to design and fabricate microarrays of different hydrogel types, fabricated with photopolymerization or thermogelation. Types of Microarrays 36 DNA Microarrays 36 Protein Microarrays 37 Tissue Microarrays 38 Cell-Based Microarrays (Transfection Microarrays) 39 Principle Steps of an Experimental Microarray Process 40 Basics 41 Surface Biofunctionalization 41 Preparation of Biomolecules (Sample Preparation) 42 3.

      Although several cell-based microarray devices and procedures for screening of chemical libraries have been reported, each has limitations in terms of simplicity, speed, and cost. Here, we sought to make a simple method for producing multiple copies of microarray chips for the controlled release of small molecules during cell-based by: 2.   Anal Bioanal Chem () – DOI /s BOOKS AND SOFTWARE IN REVIEW Alexandra Ros Published online: 12 March Springer-Verlag Bibliography the use and application of DNA microarrays. In Chapter 2, Microchip methods in diagnostics the profiling of leukaemias with the use of microarray Ursula Bilitewski (Ed.) technology is .

    It is usual to functionalize the surface of a substrate to design cell microarrays. One form of cell microarrays, the transfected cell microarray, wherein plasmid DNA or siRNA spotted on the surface of a substrate is reverse-transfected locally into adherent cells, has become a Cited by: 8. Microengineering methods for cell-based microarrays and high-throughput drug-screening applications. Feng Xu 1, JinHui Wu 1, ShuQi Wang 1, Naside Gozde Durmus 2, Umut Atakan Gurkan 1 and Utkan Demirci 1,3,4. Published 1 July • IOP Publishing Ltd Biofabrication, Volume 3, Number 3Cited by:


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Cell-based microarrays by Ella Palmer Download PDF EPUB FB2

This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays.

The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays.

About this book This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays.

Since their development incell-based microarrays have advanced significantly to include expression arrays, short interfering RNA arrays and antibody : Springer-Verlag New York. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Prools serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology Cell-based microarrays book is truly high throughput for the functional analysis of : $ This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays.

Since their development incell-based microarrays have advanced significantly to include expression arrays, short interfering RNA arrays and antibody : Springer New York. Introduction This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays.

Since their development incell-based microarrays have advanced significantly to include expression arrays, short interfering RNA arrays and antibody arrays. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of.

Cell-based microarrays: Capillary Pinning Assisted Patterning of Cell-Laden Hydrogel Microarrays in Microchips. / Gumuscu Sefunc, Burcu. New York: Humana Press, 13 p. Research output: Book/Report › Book › Academic.

from book Cell-Based Microarrays this work is the first to demonstrate the utility of surface engineered cell-based microarrays for the identification of factors that allow the selective. Book Description: As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis.

Buy Cell-Based Microarrays by Ella Palmer from Waterstones today. Click and Collect from your local Waterstones or get FREE UK delivery on orders over £/5(1). This book provides a critical analysis of the applications of microarrays in several key areas of bioanalytical research including DNA studies, peptide arrays, protein arrays, combinatorial chemistry arrays, cell-based arrays, and cturer: Springer.

Dear Colleagues, InZiauddin and Sabatini introduced the first cell-based microarray as a microarray-driven gene expression system. In the original set-up, different cDNA vectors were printed on a glass support.

After, cells were grown over the whole surface, thus resulting in a cellular response in the printed locations. Single-cell analysis provides fundamental information on individual cell response to different environmental cues and is a growing interest in cancer and stem cell research.

However, current existing methods are still facing challenges in performing such analysis in a high-throughput manner whilst being cost-effective. Here we established the Droplet Microarray (DMA) as a miniaturized Cited by: 9. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of.

This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays. Since their development incell-based microarrays have advanced signi?cantly to include expression arrays, short.

We have developed an immunofluorescence-based assay for high-throughput analysis of target proteins on a three-dimensional cellular microarray platform. This process integrates the use of three-dimensional cellular microarrays, which should better mimic the cellular microenvironment, with sensitive immunofluorescence detection and provides quantitative information on cell function.

To Cited by: This chapter reviews the development and the application of siRNA cell-based microarrays. Starting from reverse transfection as a common ground, siRNA cell-based microarrays quickly evolved into.

This tutorial review introduces the uninitiated to the world of microarrays (or so-called chips) and covers a number of basic concepts such as substrates and surfaces, printing and analysis.

It then moves on to look at some newer applications of microarray technology, which include enzyme analysis (notably kinases and proteases) as well as the growing enchantment with so-called cell-based. High-throughput adaptations of these assays, known as living-cell microarrays, which are based on microtiter plates, high-density spotting, microfabrication, and microfluidics technologies, are being developed for two general applications: (a) to screen large-scale chemical and genomic libraries and (b) to systematically investigate the local Cited by: This review focuses on the potential of high-throughput cell-based microarrays to analyze the consequences of perturbing cells with drugs, genes and other molecular cues, such as extracellular matrix (ECM) proteins, biopolymers or RNA interference (RNAi).Cited by:.

Cell-based microarrays are being increasingly used as a tool for combinatorial and high throughput screening of cellular microenvironments. Analysis of microarrays requires several steps, including microarray imaging, identification of cell spots, quality control, and data exploration.

While high content image analysis, cell counting, and cell pattern recognition methods are established, there Cited by: Cell-based microarrays are powerful analysis tools for high-throughput testing of many target samples.

Miniaturization allows the increase of assay throughput, reduction of reagent consumption and the number of cells required, thus making microarray system attractive for a variety of analysis such as drug discovery, biosensing, and by: P. Ertl, M. Rothbauer: "Cell-Based Microarrays"; in Buchreihe "Methods in Molecular Biology", Buchreihen-Herausgeber: J.

Walker; herausgegeben von: Humana Press.